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Flowcytometry in MDS and MM

Myelodysplastic Syndromes When the diagnosis of acute leukaemia or LPDs was excluded, the flow-cytometry allows to evaluate the presence of immunophenotypic lineage- associated dysplasia. From the working conference of LeukemiaNet on the flow Cytometry of Myelodisplastic Syndromes (van de Loosdrecht et al, 2009) have been identified the following features of progenitor cells as the most relevant: […]

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Sysmex XE-2100: instrument report

The Sysmex XE -2100 hematology system is a flow cytometer that uses the principle of electrical impedance associated with an optical system based on a beam of monochromatic light emitted by a semi-conductor laser. The counts and volumetric measurements of RBCs and PLTs are obtained with the impedance, or resistive, detection method. The system uses […]

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Siemens ADVIA 2120: instrument report

The technology of the Siemens ADVIA system represents the most recent development and improvement of automated flow cytochemistry, a method firstly introduced in 1975. The system includes four analytical channels, respectively dedicated to measurements of hemoglobin concentration, RBCs and PLTs, WBC total and differential count after cytochemical staining for myeloperoxidase, and count of basophil granulocytes […]

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Beckman Coulter LH series: instrument report

The Coulter LH750 system measures the count and size of leukocytes, erythrocytes and PLTs using the new AccuCount method, that integrates the Coulter impedance principle with new technologies and software algorithms with histogram distributions. In the flow-cell of the RBC/PLT channel, all electrical pulses with volume between 36 and 360 fL that are considered valid […]

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Abbott Celldyn Sapphire: instrument report

The Cell Dyn blood cell counters basically exploit a twofold methodological approach: electrical impedance and optical measurement. They count cells by electrical impedance and classify leukocytes by measuring the diffusion of a laser light beam at three different angles: 0 °, proportional to cell size; 10 °, proportional to the structure; 90 °, proportional to […]

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New instrumental parameters

As far as the erythroid series is concerned, the currently available peripheral blood parameters supply laboratory personnel with an effective screening for the differential diagnosis of anemias based on the measurement of mean cell volume (MCV), as well as on the assessment of regenerative capability (evaluation of reticulocytes and immature reticulocyte fraction) (Figures 1 to […]

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